High frequency transduction of R-factor encoded gentamicin resistance by bacteriophage P1Cm.

High-Frequency Plasmid Transduction by Lactobacillus gasseri Bacteriophage phiadh.

The temperate bacteriophage phiadh mediates plasmid DNA transduction in Lactobacillus gasseri ADH at frequencies in the range of 10 to 10 transductants per PFU. BglII-generated DNA fragments from phage phiadh were cloned into the BclI site of the transducible plasmid vector pGK12 (4.4 kb). Phage phiadh lysates induced from Lactobacillus lysogens harboring pGK12 or the recombinant plasmids were ...

Gentamicin resistance in Pseudomonas aeruginosa: R-factor-mediated resistance.

By disk diffusion antimicrobial susceptibility testing, 11% of 313 consecutive strains of Pseudomonas aeruginosa, examined during July to October 1973, were resistant to gentamicin (minimal inhibitory concentration 12.5 to >100 mug/ml), and a further 31% were moderately resistant (6.25 to 12.5 mug/ml) to gentamicin at the University of Alberta Hospital in Edmonton, Canada. Of 45 gentamicin-resi...

Transduction of R factors by a Proteus mirabilis bacteriophage.

A transducing phage, designated phim, was isolated from a lysogenic strain of Proteus mirabilis and was characterized with respect to its physical and genetic properties. The phage contains double-stranded deoxyribonucleic acid (DNA) with an S(20,w) degrees of 29 which corresponds to a molecular weight of 24 x 10(6) daltons. The base composition of phim DNA was estimated to be 40% guanine plus ...

φ Bacteriophage Lactobacillus gasseri High - Frequency Plasmid Transduction

Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22.

Transduction of R factor 222, which carries four drug resistance markers (~0, strR, camR, totR), by phage P22 in Salmonella typhimurium results in the segregation of the drug resistance markers into tetR and the other three. The tetR marker was found to be integrated at proA near the P22 prophage attachment site of the recipient chromosome, confirming the previous result of Dubnau and Stocker (...